휴먼(Human) STK40 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

STK40 cDNA Clone Datasheet

STK40 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_032017.1
• cDNA Size: 1308
• Gene Synonym: SHIK; SgK495; MGC4796; RP11-268J15.4
• Source: Human

STK40 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens serine/threonine kinase 40 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

STK40 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

STK40 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

STK40 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCGGA GAGCATCAGA CAGAGGAGCT GGGGAAACGT CGGCCAGGGC CAAGGCTCTA
61       GGAAGTGGGA TTTCTGGAAA TAATGCAAAG AGAGCTGGAC CATTCATCCT TGGTCCCCGT
121      CTGGGCAACT CACCGGTGCC AAGCATAGTG CAGTGTTTGG CGAGGAAAGA TGGCACGGAT
181      GACTTCTATC AGCTGAAGAT CCTGACCCTG GAGGAGAGGG GGGACCAAGG CATAGAGAGC
241      CAGGAAGAGC GGCAGGGCAA GATGCTGCTG CACACCGAGT ACTCACTGCT GTCTCTCCTG
301      CACACGCAGG ATGGCGTGGT GCACCACCAC GGCCTCTTCC AGGACCGCAC CTGTGAAATC
361      GTTGAGGACA CAGAATCCAG CCGGATGGTT AAGAAGATGA AGAAGCGCAT CTGCCTCGTC
421      CTGGACTGCC TCTGTGCTCA TGACTTCAGC GATAAGACCG CTGACCTCAT CAACCTGCAG
481      CACTACGTCA TCAAGGAGAA GAGGCTCAGC GAGAGGGAGA CTGTGGTAAT CTTCTACGAC
541      GTGGTCCGCG TGGTGGAGGC CCTGCACCAG AAAAATATCG TGCACAGAGA CCTGAAGCTG
601      GGGAACATGG TGCTCAACAA GAGGACACAT CGGATAACCA TCACCAACTT CTGCCTCGGG
661      AAGCATCTGG TGAGCGAGGG GGACCTGCTG AAGGACCAGA GAGGGAGCCC TGCCTACATC
721      AGTCCCGACG TGCTCAGCGG CCGGCCGTAC CGTGGCAAGC CCAGTGACAT GTGGGCCCTG
781      GGCGTGGTGC TCTTCACCAT GCTGTATGGC CAGTTCCCCT TCTACGACAG CATCCCGCAG
841      GAGCTCTTCC GCAAGATCAA GGCTGCCGAG TATACCATTC CTGAGGATGG ACGGGTTTCT
901      GAGAACACCG TGTGTCTCAT CCGGAAGCTG CTGGTCCTTG ACCCCCAGCA GCGCCTGGCC
961      GCCGCCGACG TCCTGGAGGC CCTCAGTGCC ATCATTGCAT CATGGCAGTC CCTGTCATCT
1021     CTGAGTGGGC CTTTGCAAGT GGTTCCTGAC ATTGATGACC AAATGAGCAA TGCGGATAGC
1081     TCCCAGGAGG CGAAGGTGAC GGAGGAGTGC TCCCAGTACG AGTTTGAGAA CTACATGCGT
1141     CAGCAGCTGC TGCTGGCCGA GGAGAAGAGC TCCATCCATG ACGCCCGGAG CTGGGTACCC
1201     AAGCGGCAGT TCGGCAGCGC ACCACCGGTG CGACGGCTGG GCCACGACGC ACAGCCCATG
1261     ACCTCCTTGG ACACGGCCAT CCTGGCGCAG CGCTACCTGC GGAAATAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).