마우스(Mouse) SERPIND1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

SERPIND1 cDNA Clone Datasheet

SERPIND1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008223.3
• cDNA Size: 1437
• Gene Synonym: HCII; Hcf2; AA985900; AI303446; MGC107662
• Source: Mouse

SERPIND1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serine (or cysteine) peptidase inhibitor, clade D, member 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SERPIND1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SERPIND1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

SERPIND1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAACATC CACTCTGCAC TCTTCTTTCT CTCATCACAT TTATGTGTAT TGGGAGCAAA
61       GGCCTTGCTG AGCAGCTAAC TAATGAAAAC CTGACCACGT CCTTCCTGCC TGCTAACTTC
121      CACAAAGAAA ACACAGTCAC CAATGACTGG ATCCCAGAGG GGGAAGAGGA TGAGGACTAC
181      CTGGACCTGG AGAAGCTGCT TGGTGAAGAT GACGACTACA TTTATATCAT CGATGCCGTT
241      TCCCCGACAG ACTCAGAATC AAGTGCTGGC AACATCCTGC AGCTTTTCCA AGGCAAGAGC
301      CGGATCCAGC GTCTTAATAT CCTCAATGCA AAGTTTGCCT TCAACCTTTA CCGAGTCCTG
361      AAGGACCAGG CTACCACATC TGATAACCTC TTCATAGCAC CTGTTGGCAT TTCTACTGCC
421      ATGGGGATGA TCTCCTTAGG TCTGAGGGGA GAGACCCATG AAGAAGTACA CTCAGTTCTA
481      CACTTCAGAG ACTTTGTCAA TGCTAGCAGC AAGTACGAGG TTACCACCAT TCACAATCTC
541      TTTCGAAAGC TGACCCATCG TCTATTCAGG AGGAATTTTG GGTACACACT TCGGTCAGTT
601      AATGGCCTTT ATATTCAGAA GCAGTTTCCC ATACGGGAGG ACTTCAAAGC TGCTATGAGA
661      GAGTTTTACT TTGCTGAGGC CCAGGAGGCT AACTTCCCAG ATCCTGCCTT CATATCAAAG
721      GCAAACAACC ACATTCTGAA ACTCACCAAG GGCCTTATAA AAGAAGCTCT GGAGAATATA
781      GATCCTGCTA CTCAGATGCT GATTCTGAAC TGCATCTACT TCAAAGGAAC TTGGGTGAAT
841      AAATTCCCAG TAGAAATGAC GCACAACCAC AACTTCCGGC TGAATGAGAG AGAAGTAGTC
901      AAAGTCTCCA TGATGCAAAC TAAAGGGAAT TTCCTTGCGG CAAATGACCA GGAGCTAGAC
961      TGTGACATTC TCCAGCTGGA GTACGTAGGG GGCATCAGCA TGCTTATTGT AGTTCCACGG
1021     AAGCTATCGG GAATGAAGAC TCTTGAAGCA CAGCTTACAC CCCAGGTGGT GGAGAGATGG
1081     CAAAAAAGCA TGACAAACAG AACTCGAGAG GTACTTCTGC CAAAGTTTAA GCTTGAGAAG
1141     AATTACAACC TGGTGGAGGT CCTTAAGTCC ATGGGAATCA CAAAGCTCTT CAACAAGAAT
1201     GGCAATATGT CAGGCATCTC AGACCAGAGG ATCGCCATTG ACCTGTTCAA GCACCAAAGT
1261     ACCATCACAG TGAATGAAGA GGGTACCCAA GCTGCAGCTG TAACTACAGT GGGGTTCATG
1321     CCGCTGTCTA CCCAAGTCCG ATTCACTGTC GACCGTCCCT TCCTGTTCCT AGTGTATGAG
1381     CACCGAACCA GCTGTCTGCT CTTCATGGGA AAAGTGACCA ACCCTGCCAA GTCTTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).