마우스(Mouse) APCS / SAP 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

APCS cDNA Clone Datasheet

APCS cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011318.1
• cDNA Size: 675
• Gene Synonym: Sap
• Source: Mouse

APCS cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serum amyloid P-component DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 480 C/T not causing the amino acid variation.

APCS cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

APCS cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

APCS cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACAAGC TACTGCTTTG GATGTTTGTC TTCACCAGCC TTCTTTCAGA AGCCTTTTGT
61       CAGACAGACC TCAAGAGGAA AGTATTTGTG TTCCCCAGAG AATCTGAAAC TGATCATGTG
121      AAGCTGATCC CACATCTAGA GAAACCTCTG CAGAATTTTA CACTGTGTTT CCGAACCTAC
181      AGTGACCTTT CCCGCTCTCA GAGTCTTTTC TCCTACAGTG TCAAGGGCAG AGACAATGAG
241      CTACTAATTT ATAAAGAAAA AGTTGGAGAA TACAGCCTAT ACATCGGACA ATCAAAAGTC
301      ACAGTCCGTG GTATGGAAGA ATACCTTTCT CCAGTACACC TATGTACCAC TTGGGAGTCC
361      TCCTCTGGCA TTGTTGAATT TTGGGTCAAT GGAAAGCCTT GGGTAAAAAA GTCTCTGCAG
421      AGGGAATACA CTGTGAAAGC CCCACCCAGT ATAGTCCTGG GACAGGAGCA GGATAACTAT
481      GGAGGAGGGT TTCAAAGGTC ACAGTCCTTT GTAGGAGAGT TTTCAGATTT ATACATGTGG
541      GACTATGTGC TGACCCCACA AGACATTCTA TTTGTGTACA GAGATTCCCC TGTCAATCCT
601      AATATTTTGA ATTGGCAGGC TCTTAACTAT GAAATAAATG GCTACGTAGT CATCAGGCCC
661      CGTGTCTGGG ATTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).