휴먼(Human) Sonic Hedgehog 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10372-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000193.2

cDNA Size:   1389

Synonym:  TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5,SHH

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens sonic hedgehog homolog (Drosophila) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCTGCTGC TGGCGAGATG TCTGCTGCTA GTCCTCGTCT CCTCGCTGCT GGTATGCTCG
61       GGACTGGCGT GCGGACCGGG CAGGGGGTTC GGGAAGAGGA GGCACCCCAA AAAGCTGACC
121      CCTTTAGCCT ACAAGCAGTT TATCCCCAAT GTGGCCGAGA AGACCCTAGG CGCCAGCGGA
181      AGGTATGAAG GGAAGATCTC CAGAAACTCC GAGCGATTTA AGGAACTCAC CCCCAATTAC
241      AACCCCGACA TCATATTTAA GGATGAAGAA AACACCGGAG CGGACAGGCT GATGACTCAG
301      AGGTGTAAGG ACAAGTTGAA CGCTTTGGCC ATCTCGGTGA TGAACCAGTG GCCAGGAGTG
361      AAACTGCGGG TGACCGAGGG CTGGGACGAA GATGGCCACC ACTCAGAGGA GTCTCTGCAC
421      TACGAGGGCC GCGCAGTGGA CATCACCACG TCTGACCGCG ACCGCAGCAA GTACGGCATG
481      CTGGCCCGCC TGGCGGTGGA GGCCGGCTTC GACTGGGTGT ACTACGAGTC CAAGGCACAT
541      ATCCACTGCT CGGTGAAAGC AGAGAACTCG GTGGCGGCCA AATCGGGAGG CTGCTTCCCG
601      GGCTCGGCCA CGGTGCACCT GGAGCAGGGC GGCACCAAGC TGGTGAAGGA CCTGAGCCCC
661      GGGGACCGCG TGCTGGCGGC GGACGACCAG GGCCGGCTGC TCTACAGCGA CTTCCTCACT
721      TTCCTGGACC GCGACGACGG CGCCAAGAAG GTCTTCTACG TGATCGAGAC GCGGGAGCCG
781      CGCGAGCGCC TGCTGCTCAC CGCCGCGCAC CTGCTCTTTG TGGCGCCGCA CAACGACTCG
841      GCCACCGGGG AGCCCGAGGC GTCCTCGGGC TCGGGGCCGC CTTCCGGGGG CGCACTGGGG
901      CCTCGGGCGC TGTTCGCCAG CCGCGTGCGC CCGGGCCAGC GCGTGTACGT GGTGGCCGAG
961      CGTGACGGGG ACCGCCGGCT CCTGCCCGCC GCTGTGCACA GCGTGACCCT AAGCGAGGAG
1021     GCCGCGGGCG CCTACGCGCC GCTCACGGCC CAGGGCACCA TTCTCATCAA CCGGGTGCTG
1081     GCCTCGTGCT ACGCGGTCAT CGAGGAGCAC AGCTGGGCGC ACCGGGCCTT CGCGCCCTTC
1141     CGCCTGGCGC ACGCGCTCCT GGCTGCACTG GCGCCCGCGC GCACGGACCG CGGCGGGGAC
1201     AGCGGCGGCG GGGACCGCGG GGGCGGCGGC GGCAGAGTAG CCCTAACCGC TCCAGGTGCT
1261     GCCGACGCTC CGGGTGCGGG GGCCACCGCG GGCATCCACT GGTACTCGCA GCTGCTCTAC
1321     CAAATAGGCA CCTGGCTCCT GGACAGCGAG GCCCTGCACC CGCTGGGCAT GGCGGTCAAG
1381     TCCAGCTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) Sonic Hedgehog 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)