SDC1 / CD138 단백질(Protein) (His Tag)

Syndecan-1 / SDC1 / CD138 Protein

Syndecan-1 / SDC1 / CD138 Protein Product Information

• Synonym: CD138, SDC, SYND1, syndecan
• Protein Construction: A DNA sequence encoding the human SDC1 ( NP_002988.3 ) extracellular domain ( Met 1 - Glu 251 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

Syndecan-1 / SDC1 / CD138 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 18
• Molecular Mass: The recombinant human SDC1 consists of 245 amino acids and predictes a molecular mass of 25.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SDC1 is approximately 48-55 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: SDC1 protein

Syndecan-1 / SDC1 / CD138 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Syndecan-1 / SDC1 / CD138 Protein Description

Syndecan-1, also known as SYND1, CD138 and SDC1, is a single-pass type I membrane protein which belongs to the syndecan proteoglycan family. CD138 / Syndecan-1, is well known to be associated with cell proliferation, adhesion, and migration in various types of malignancies. CD138 / Syndecan-1 is a cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. It is a cell-surface heparan sulfate proteoglycan expressed on most epithelial cells, and decreased CD138 expression is associated with increased invasive and metastatic potential in carcinomas. Within the hematopoietic system, CD138 is an excellent marker of plasmacytic differentiation. CD138 / Syndecan-1 is a cell surface proteoglycan that binds cells to the extracellular matrix and its expression is down-regulated in many cellular transformation models. CD138 / Syndecan-1 is a new simple non-invasive marker for predicting liver fibrosis in patients with chronic hepatitis C. The relevance of this marker in combination with other fibrosis markers should be explored. CD138 / Syndecan-1 might contribute to urothelial carcinoma cell survival and progression. It could be a new therapeutic target in human urinary bladder cancer.

References

1. O'Connell, FP. et al., Am J Clin Pathol 2004, 121 (2):254-63.
2. Gökden,N.et al., 2006, Appl Immunohistochem Mol Morphol. 14 (2): 173 - 7.
3. Sun,WP. et al., 2007,Cell Mol Immunol. 4 (3): 209-14.
4. Zvibel, I. et al., 2009, Liver Int  29 (2): 208-12.
5. Shimada,K. et al., 2010, Cancer Sci. 101 (1):155-60.