마우스(Mouse) TNFRSF13B 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

TNFRSF13B cDNA Clone Datasheet

TNFRSF13B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021349.1
• cDNA Size: 750
• Gene Synonym: Taci; 1200009E08Rik
• Source: Mouse

TNFRSF13B cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tumor necrosis factor receptor superfamily, member 13b DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TNFRSF13B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFRSF13B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TNFRSF13B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTATGG CATTCTGCCC CAAAGATCAG TACTGGGACT CCTCAAGGAA ATCCTGTGTC
61       TCCTGTGCAC TGACCTGCAG CCAGAGGAGC CAGCGCACCT GTACAGACTT CTGCAAATTC
121      ATCAATTGCC GAAAAGAGCA AGGCAGGTAC TACGACCATC TCCTGGGGGC CTGCGTCAGC
181      TGTGACTCCA CCTGCACACA GCACCCTCAG CAGTGTGCCC ACTTCTGTGA GAAAAGGCCC
241      AGAAGCCAGG CGAACCTCCA GCCCGAGCTC GGGAGACCAC AGGCCGGGGA GGTGGAAGTC
301      AGGTCAGACA ACTCAGGAAG GCACCAGGGA TCTGAGCATG GTCCAGGATT GAGGCTAAGT
361      AGCGACCAGC TGACTCTCTA CTGCACACTG GGGGTCTGCC TCTGCGCCAT CTTCTGCTGT
421      TTCTTGGTGG CCTTGGCCTC CTTCCTCAGG CGTAGAGGAG AGCCACTACC CAGCCAGCCT
481      GCCGGGCCAC GTGGGTCACA AGCAAACTCT CCCCACGCCC ACCGCCCCGT GACAGAGGCT
541      TGCGACGAGG TGACCGCGTC ACCCCAGCCT GTGGAAACGT GTAGCTTCTG CTTCCCGGAG
601      CGCAGTTCTC CCACTCAGGA GAGCGCGCCG CGTTCGCTCG GGATACACGG CTTCGCGGGC
661      ACTGCCGCCC CGCAGCCCTG TATGCGTGCA ACAGTAGGCG GCCTGGGTGT CCTGCGCGCA
721      TCCACTGGGG ACGCTCGTCC GGCAACTTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).