휴먼(Human) TLE1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

TLE1 cDNA Clone Datasheet

TLE1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005077.3
• cDNA Size: 2313
• Gene Synonym: ESG; ESG1; GRG1; TLE1
• Source: Human

TLE1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the mutation 354 A/G not causing the amino acid variation.

TLE1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TLE1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TLE1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC
61       ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
121      CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
181      TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
241      ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
301      CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAGTTGAAT
361      GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
421      GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
481      AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
541      GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
601      TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
661      TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
721      GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
781      CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
841      AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
901      TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
961      ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
1021     CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGCAGCTGG CTTGAGGACA
1081     CCCCTGGCAG TGCCCGGCCC ATATCCTGCT CCTTTTGGGA TGGTCCCCCA CGCTGGCATG
1141     AACGGCGAGC TGACCAGCCC AGGCGCTGCC TACGCCAGTT TACACAACAT GTCGCCCCAG
1201     ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG GTGGCCTACG GGCGCTCCCC CATGGTGGGG
1261     TTTGATCCTC CCCCTCACAT GAGAGTACCT ACCATTCCTC CAAACCTGGC AGGAATCCCT
1321     GGGGGGAAAC CTGCATACTC CTTCCACGTT ACTGCAGACG GTCAGATGCA GCCTGTCCCT
1381     TTTCCCCCCG ACGCCCTCAT CGGACCCGGA ATCCCCCGGC ATGCTCGCCA GATCAACACC
1441     CTCAACCACG GGGAGGTGGT GTGCGCTGTG ACCATCAGCA ACCCCACGAG ACACGTGTAC
1501     ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA TAAGAGCCCT
1561     GTCTCCCAGC TCGACTGTCT GAACAGAGAC AATTATATCC GTTCCTGTAA ATTGCTACCC
1621     GATGGCTGCA CTCTCATAGT GGGAGGGGAA GCCAGTACTT TGTCCATTTG GGACCTGGCG
1681     GCTCCAACCC CGCGCATCAA GGCGGAGCTG ACGTCCTCGG CCCCCGCCTG CTACGCCCTG
1741     GCCATCAGCC CCGATTCCAA GGTCTGCTTC TCATGCTGCA GCGACGGCAA CATCGCTGTG
1801     TGGGATCTGC ACAACCAGAC ACTAGTGAGG CAATTCCAGG GCCACACAGA CGGAGCCAGC
1861     TGTATTGACA TTTCTAATGA TGGCACCAAG CTCTGGACGG GTGGTTTGGA CAACACAGTC
1921     AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
1981     TTCTCCCTGG GGTACTGCCC CACCGGGGAG TGGCTGGCAG TGGGCATGGA GAGCAGCAAT
2041     GTGGAGGTGC TGCACGTGAA CAAGCCTGAC AAGTACCAGC TGCACCTGCA TGAGAGCTGC
2101     GTGCTGTCCC TGAAATTTGC TTACTGTGGT AAATGGTTTG TGAGTACTGG AAAAGATAAC
2161     CTCCTCAATG CTTGGCGGAC CCCCTATGGA GCCAGCATAT TCCAGTCCAA AGAGTCCTCG
2221     TCAGTGCTTA GCTGTGACAT CTCTGTGGAT GATAAGTACA TAGTCACTGG CTCGGGGGAC
2281     AAGAAGGCTA CAGTCTATGA AGTCATCTAC TGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).