Canine TNFa 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

TNF cDNA Clone Datasheet

TNF cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001003244.4
• cDNA Size: 702
• Gene Synonym: DIF; TNFA; TNFSF2; TNF-alpha; TNF
• Source: Canine

TNF cDNA Clone Description

• cDNA Description: ORF Clone of Canis lupus familiars tumor necrosis factor (TNF superfamily, member 2) DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

TNF cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNF cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TNF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGCCGCT CCCCAAGAAG
61       GCGGGGGGCC CCCCGGGCTC CAGAAGGTGC TTCTGCCTCA GCCTCTTCTC CTTCCTCCTC
121      GTCGCAGGGG CCACCACACT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
181      GAAGAGCTCC CAAATGGCCT CCAACTAATC AGCCCTCTTG CCCAGACAGT CAAATCATCT
241      TCTCGAACCC CAAGTGACAA GCCAGTAGCT CATGTTGTAG CAAACCCCGA AGCTGAGGGG
301      CAGCTCCAGT GGCTGAGCCG ACGTGCCAAT GCCCTCCTGG CCAACGGCGT GGAGCTGACA
361      GACAACCAGC TGATAGTGCC GTCAGATGGG TTGTACCTCA TCTACTCCCA GGTCCTCTTC
421      AAGGGCCAAG GGTGCCCTTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCTTCGCC
481      GTCTCCTACC AGACAAAGGT CAACCTACTC TCTGCCATCA AGAGCCCTTG CCAAAGGGAG
541      ACCCCAGAGG GGACCGAGGC CAAGCCCTGG TACGAGCCCA TCTACCTGGG AGGGGTCTTC
601      CAACTGGAGA AGGGTGATCG ACTCAGCGCT GAGATCAATC TGCCTAACTA TCTGGACTTT
661      GCCGAGTCCG GGCAGGTGTA CTTTGGAATC ATTGCCCTGT AA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).