Mouse TNFRSF1A / CD120a / TNFRI 단백질(Protein) (His Tag)

Mouse Tumor Necrosis Factor Receptor Superfamily Member 1A Protein

TNFRSF1A / CD120a Protein Product Information

• Synonym: Tnfrsf1, CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI
• Protein Construction: A DNA sequence encoding the mouse TNFRSF1A ( NP_ 035739.2 ) extracellular domain ( Met 1-Ala 212 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

TNFRSF1A / CD120a Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu30
• Molecular Mass: The secreted recombinant mouse TNFRSF1A consists of 194 amino acids and has a predicted molecular mass of 21.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A is approximately 35 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: TNFRSF1A protein

TNFRSF1A / CD120a Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFRSF1A / CD120a Protein Description

Mouse tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A) , also known as TNFRI, TNF R-p55/p60, and CD120a, is a type I transmembrane protein widely expressed and is present on the cell surface as a trimer. TNFRSF1A / CD120a contains one death domain and four TNFR-Cys repeats. The aggregated death domains of TNFRSF1A provide a novel molecular interface that interacts specifically with the death domain of TRADD. TNFRSF1A serves as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor, and the resulting death-inducing signaling complex (DISC) initiates the subsequent cascade of caspases mediating apoptosis. TNFRI can also activate the transcription factor NF-kB, and functions as a regulator of inflammation. Germline mutations of the extracellular domains of TNF RI are found to be associated with the human genetic disorder called periodic fever syndrome. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) which is a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia. Reactive amyloidosis is the main complication and occurs in 25% of cases.

References

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5. Micheau, O. et al., 2003, Cell. 114 (2): 181-90.
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