마우스(Mouse) TNFRSF1B 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

TNFRSF1B cDNA Clone Datasheet

TNFRSF1B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011610.3
• cDNA Size: 1425
• Gene Synonym: p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2
• Source: Mouse

TNFRSF1B cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tumor necrosis factor receptor superfamily, member 1b DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 6 G/A not causing the amino acid variation.

TNFRSF1B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFRSF1B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TNFRSF1B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCACCCG CCGCCCTCTG GGTCGCGCTG GTCTTCGAAC TGCAGCTGTG GGCCACCGGG
61       CACACAGTGC CCGCCCAGGT TGTCTTGACA CCCTACAAAC CGGAACCTGG GTACGAGTGC
121      CAGATCTCAC AGGAATACTA TGACAGGAAG GCTCAGATGT GCTGTGCTAA GTGTCCTCCT
181      GGCCAATATG TGAAACATTT CTGCAACAAG ACCTCGGACA CCGTGTGTGC GGACTGTGAG
241      GCAAGCATGT ATACCCAGGT CTGGAACCAG TTTCGTACAT GTTTGAGCTG CAGTTCTTCC
301      TGTACCACTG ACCAGGTGGA GATCCGCGCC TGCACTAAAC AGCAGAACCG AGTGTGTGCT
361      TGCGAAGCTG GCAGGTACTG CGCCTTGAAA ACCCATTCTG GCAGCTGTCG ACAGTGCATG
421      AGGCTGAGCA AGTGCGGCCC TGGCTTCGGA GTGGCCAGTT CAAGAGCCCC AAATGGAAAT
481      GTGCTATGCA AGGCCTGTGC CCCAGGGACG TTCTCTGACA CCACATCATC CACTGATGTG
541      TGCAGGCCCC ACCGCATCTG TAGCATCCTG GCTATTCCCG GAAATGCAAG CACAGATGCA
601      GTCTGTGCGC CCGAGTCCCC AACTCTAAGT GCCATCCCAA GGACACTCTA CGTATCTCAG
661      CCAGAGCCCA CAAGATCCCA ACCCCTGGAT CAAGAGCCAG GGCCCAGCCA AACTCCAAGC
721      ATCCTTACAT CGTTGGGTTC AACCCCCATT ATTGAACAAA GTACCAAGGG TGGCATCTCT
781      CTTCCAATTG GTCTGATTGT TGGAGTGACA TCACTGGGTC TGCTGATGTT AGGACTGGTG
841      AACTGCATCA TCCTGGTGCA GAGGAAAAAG AAGCCCTCCT GCCTACAAAG AGATGCCAAG
901      GTGCCTCATG TGCCTGATGA GAAATCCCAG GATGCAGTAG GCCTTGAGCA GCAGCACCTG
961      TTGACCACAG CACCCAGTTC CAGCAGCAGC TCCCTAGAGA GCTCAGCCAG CGCTGGGGAC
1021     CGAAGGGCGC CCCCTGGGGG CCATCCCCAA GCAAGAGTCA TGGCGGAGGC CCAAGGGTTT
1081     CAGGAGGCCC GTGCCAGCTC CAGGATTTCA GATTCTTCCC ACGGAAGCCA CGGGACCCAC
1141     GTCAACGTCA CCTGCATCGT GAACGTCTGT AGCAGCTCTG ACCACAGTTC TCAGTGCTCT
1201     TCCCAAGCCA GCGCCACAGT GGGAGACCCA GATGCCAAGC CCTCAGCGTC CCCAAAGGAT
1261     GAGCAGGTCC CCTTCTCTCA GGAGGAGTGT CCGTCTCAGT CCCCGTGTGA GACTACAGAG
1321     ACACTGCAGA GCCATGAGAA GCCCTTGCCC CTTGGTGTGC CGGATATGGG CATGAAGCCC
1381     AGCCAAGCTG GCTGGTTTGA TCAGATTGCA GTCAAAGTGG CCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).