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휴먼(Human) TNFRSF12 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)PDF Download

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HG10431-M
  YES       
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_016639.2

cDNA Size:   390

Synonym:  FN14; CD266; TWEAKR; TNFRSF12A

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens tumor necrosis factor receptor superfamily, member 12A DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
  1. Remove the marked edge in the center of the spot .
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGCTCGGG GCTCGCTGCG CCGGTTGCTG CGGCTCCTCG TGCTGGGGCT CTGGCTGGCG
61 TTGCTGCGCT CCGTGGCCGG GGAGCAAGCG CCAGGCACCG CCCCCTGCTC CCGCGGCAGC
121 TCCTGGAGCG CGGACCTGGA CAAGTGCATG GACTGCGCGT CTTGCAGGGC GCGACCGCAC
181 AGCGACTTCT GCCTGGGCTG CGCTGCAGCA CCTCCTGCCC CCTTCCGGCT GCTTTGGCCC
241 ATCCTTGGGG GCGCTCTGAG CCTGACCTTC GTGCTGGGGC TGCTTTCTGG CTTTTTGGTC
301 TGGAGACGAT GCCGCAGGAG AGAGAAGTTC ACCACCCCCA TAGAGGAGAC CGGCGGAGAG
361 GGCTGCCCAG CTGTGGCGCT GATCCAGTGA

Quality Control:   cDNA sequencing results is available upon request.


Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).