휴먼(Human) TNFSF10/TRAIL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_003810.2

cDNA Size:   846

Synonym:  TL2, APO2L, CD253, TRAIL, Apo-2L, TNFSF10

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 825C/T not causing the amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGCTATGA TGGAGGTCCA GGGGGGACCC AGCCTGGGAC AGACCTGCGT GCTGATCGTG
61       ATCTTCACAG TGCTCCTGCA GTCTCTCTGT GTGGCTGTAA CTTACGTGTA CTTTACCAAC
121      GAGCTGAAGC AGATGCAGGA CAAGTACTCC AAAAGTGGCA TTGCTTGTTT CTTAAAAGAA
181      GATGACAGTT ATTGGGACCC CAATGACGAA GAGAGTATGA ACAGCCCCTG CTGGCAAGTC
241      AAGTGGCAAC TCCGTCAGCT CGTTAGAAAG ATGATTTTGA GAACCTCTGA GGAAACCATT
301      TCTACAGTTC AAGAAAAGCA ACAAAATATT TCTCCCCTAG TGAGAGAAAG AGGTCCTCAG
361      AGAGTAGCAG CTCACATAAC TGGGACCAGA GGAAGAAGCA ACACATTGTC TTCTCCAAAC
421      TCCAAGAATG AAAAGGCTCT GGGCCGCAAA ATAAACTCCT GGGAATCATC AAGGAGTGGG
481      CATTCATTCC TGAGCAACTT GCACTTGAGG AATGGTGAAC TGGTCATCCA TGAAAAAGGG
541      TTTTACTACA TCTATTCCCA AACATACTTT CGATTTCAGG AGGAAATAAA AGAAAACACA
601      AAGAACGACA AACAAATGGT CCAATATATT TACAAATACA CAAGTTATCC TGACCCTATA
661      TTGTTGATGA AAAGTGCTAG AAATAGTTGT TGGTCTAAAG ATGCAGAATA TGGACTCTAT
721      TCCATCTATC AAGGGGGAAT ATTTGAGCTT AAGGAAAATG ACAGAATTTT TGTTTCTGTA
781      ACAAATGAGC ACTTGATAGA CATGGACCAT GAAGCCAGTT TTTTTGGGGC CTTTTTAGTT
841      GGCTAA

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).