휴먼(Human) TRAILR1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10408-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_003844.2

cDNA Size:   1407

Synonym:  DR4, APO2, CD261, MGC9365, TRAILR1, TRAILR-1, TNFRSF10A

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens tumor necrosis factor receptor superfamily, member 10a (TNFRSF10A) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 683A/C causing the encoded amino acid 228Glu to be substituted by Ala.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGCGCCAC CACCAGCTAG AGTACATCTA GGTGCGTTCC TGGCAGTGAC TCCGAATCCC
61       GGGAGCGCAG CGAGTGGGAC AGAGGCAGCC GCGGCCACAC CCAGCAAAGT GTGGGGCTCT
121      TCCGCGGGGA GGATTGAACC ACGAGGCGGG GGCCGAGGAG CGCTCCCTAC CTCCATGGGA
181      CAGCACGGAC CCAGTGCCCG GGCCCGGGCA GGGCGCGCCC CAGGACCCAG GCCGGCGCGG
241      GAAGCCAGCC CTCGGCTCCG GGTCCACAAG ACCTTCAAGT TTGTCGTCGT CGGGGTCCTG
301      CTGCAGGTCG TACCTAGCTC AGCTGCAACC ATCAAACTTC ATGATCAATC AATTGGCACA
361      CAGCAATGGG AACATAGCCC TTTGGGAGAG TTGTGTCCAC CAGGATCTCA TAGATCAGAA
421      CATCCTGGAG CCTGTAACCG GTGCACAGAG GGTGTGGGTT ACACCAATGC TTCCAACAAT
481      TTGTTTGCTT GCCTCCCATG TACAGCTTGT AAATCAGATG AAGAAGAGAG AAGTCCCTGC
541      ACCACGACCA GGAACACAGC ATGTCAGTGC AAACCAGGAA CTTTCCGGAA TGACAATTCT
601      GCTGAGATGT GCCGGAAGTG CAGCAGAGGG TGCCCCAGAG GGATGGTCAA GGTCAAGGAT
661      TGTACGCCCT GGAGTGACAT CGCGTGTGTC CACAAAGAAT CAGGCAATGG ACATAATATA
721      TGGGTGATTT TGGTTGTGAC TTTGGTTGTT CCGTTGCTGT TGGTGGCTGT GCTGATTGTC
781      TGTTGTTGCA TCGGCTCAGG TTGTGGAGGG GACCCCAAGT GCATGGACAG GGTGTGTTTC
841      TGGCGCTTGG GTCTCCTACG AGGGCCTGGG GCTGAGGACA ATGCTCACAA CGAGATTCTG
901      AGCAACGCAG ACTCGCTGTC CACTTTCGTC TCTGAGCAGC AAATGGAAAG CCAGGAGCCG
961      GCAGATTTGA CAGGTGTCAC TGTACAGTCC CCAGGGGAGG CACAGTGTCT GCTGGGACCG
1021     GCAGAAGCTG AAGGGTCTCA GAGGAGGAGG CTGCTGGTTC CAGCAAATGG TGCTGACCCC
1081     ACTGAGACTC TGATGCTGTT CTTTGACAAG TTTGCAAACA TCGTGCCCTT TGACTCCTGG
1141     GACCAGCTCA TGAGGCAGCT GGACCTCACG AAAAATGAGA TCGATGTGGT CAGAGCTGGT
1201     ACAGCAGGCC CAGGGGATGC CTTGTATGCA ATGCTGATGA AATGGGTCAA CAAAACTGGA
1261     CGGAACGCCT CGATCCACAC CCTGCTGGAT GCCTTGGAGA GGATGGAAGA GAGACATGCA
1321     AAAGAGAAGA TTCAGGACCT CTTGGTGGAC TCTGGAAAGT TCATCTACTT AGAAGATGGC
1381     ACAGGCTCTG CCGTGTCCTT GGAGTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) TRAILR1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)