휴먼(Human) TUBB 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

TUBB cDNA Clone Datasheet

TUBB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_178014.2
• cDNA Size: 1335
• Gene Synonym: M40; TUBB1; TUBB5; MGC16435; MGC117247; OK/SW-cl.56; TUBB
• Source: Human

TUBB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens tubulin, beta DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TUBB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TUBB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TUBB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGAAA TCGTGCACAT CCAGGCTGGT CAGTGTGGCA ACCAGATCGG TGCCAAGTTC
61       TGGGAGGTGA TCAGTGATGA ACATGGCATC GACCCCACCG GCACCTACCA CGGGGACAGC
121      GACCTGCAGC TGGACCGCAT CTCTGTGTAC TACAATGAAG CCACAGGTGG CAAATATGTT
181      CCTCGTGCCA TCCTGGTGGA TCTAGAACCT GGGACCATGG ACTCTGTTCG CTCAGGTCCT
241      TTTGGCCAGA TCTTTAGACC AGACAACTTT GTATTTGGTC AGTCTGGGGC AGGTAACAAC
301      TGGGCCAAAG GCCACTACAC AGAGGGCGCC GAGCTGGTTG ATTCTGTCCT GGATGTGGTA
361      CGGAAGGAGG CAGAGAGCTG TGACTGCCTG CAGGGCTTCC AGCTGACCCA CTCACTGGGC
421      GGGGGCACAG GCTCTGGAAT GGGCACTCTC CTTATCAGCA AGATCCGAGA AGAATACCCT
481      GATCGCATCA TGAATACCTT CAGTGTGGTG CCTTCACCCA AAGTGTCTGA CACCGTGGTC
541      GAGCCCTACA ATGCCACCCT CTCCGTCCAT CAGTTGGTAG AGAATACTGA TGAGACCTAT
601      TGCATTGACA ACGAGGCCCT CTATGATATC TGCTTCCGCA CTCTGAAGCT GACCACACCA
661      ACCTACGGGG ATCTGAACCA CCTTGTCTCA GCCACCATGA GTGGTGTCAC CACCTGCCTC
721      CGTTTCCCTG GCCAGCTCAA TGCTGACCTC CGCAAGTTGG CAGTCAACAT GGTCCCCTTC
781      CCACGTCTCC ATTTCTTTAT GCCTGGCTTT GCCCCTCTCA CCAGCCGTGG AAGCCAGCAG
841      TATCGAGCTC TCACAGTGCC GGAACTCACC CAGCAGGTCT TCGATGCCAA GAACATGATG
901      GCTGCCTGTG ACCCCCGCCA CGGCCGATAC CTCACCGTGG CTGCTGTCTT CCGTGGTCGG
961      ATGTCCATGA AGGAGGTCGA TGAGCAGATG CTTAACGTGC AGAACAAGAA CAGCAGCTAC
1021     TTTGTGGAAT GGATCCCCAA CAATGTCAAG ACAGCCGTCT GTGACATCCC ACCTCGTGGC
1081     CTCAAGATGG CAGTCACCTT CATTGGCAAT AGCACAGCCA TCCAGGAGCT CTTCAAGCGC
1141     ATCTCGGAGC AGTTCACTGC CATGTTCCGC CGGAAGGCCT TCCTCCACTG GTACACAGGC
1201     GAGGGCATGG ACGAGATGGA GTTCACCGAG GCTGAGAGCA ACATGAACGA CCTCGTCTCT
1261     GAGTATCAGC AGTACCAGGA TGCCACCGCA GAAGAGGAGG AGGATTTCGG TGAGGAGGCC
1321     GAAGAGGAGG CCTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).