Anti-Transferrin Receptor Antibody

Cat: 11020-R001

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Anti-Transferrin Receptor Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Transferrin Receptor Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Transferrin Receptor
Immunogen
Recombinant Human Transferrin Receptor / TFRC / CD71 protein (Catalog#11020-H07H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; Catalog#11020-H07H; NP_003225.2; Cys89-Phe 760).
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-Transferrin Receptor Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Transferrin Receptor Antibody (Rabbit Monoclonal antibody) Images

Human TFRC/CD71 Western blot (WB) 13324

Anti-TFRC rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:84 kDa

Observed band size:95 kDa

Human TFRC/CD71 Immunoprecipitation(IP) 17680

TFRC was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

Lane C:0.5 mg HepG2 Whole Cell Lysate

Lane D:0.5 mg Hela Whole Cell Lysate

2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TFRC rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 84 kDa

Observed band size: 100 kDa

Human Transferrin Receptor Western blot (WB) 19435

Anti-TFRC rabbit monoclonal antibody at 1:500 dilution

Lane A: TFRC konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:84 kDa

Observed band size:85 kDa

Anti-Transferrin Receptor Antibody Alternative Names

Anti-CD71 Antibody;Anti-p90 Antibody;Anti-T9 Antibody;Anti-TFR Antibody;Anti-TFR1 Antibody;Anti-TR Antibody;Anti-TRFR Antibody

Transferrin Receptor Background Information

Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p90, CD71 and TFRC, is a single-pass type II membrane protein which belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.

Full Name
transferrin receptor
References
  • Douabin-Gicquel V., et al., 2001,Hum. Genet. 109:393-401.
  • Ryschich,E. et al., 2004,Eur J Cancer. 40 (9):1418-22.
  • Tosoni D., et al., 2005, Cell 123:875-888.
  • Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
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