Anti-UCHL1 Antibody

Cat: 80089-RP02

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Anti-UCHL1 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-UCHL1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Rat
Specificity
Rat UCHL1
Immunogen
Recombinant Rat UCHL1 protein (Catalog#80089-R07E)
Preparation
Produced in rabbits immunized with purified, recombinant Rat UCHL1 (rR UCHL1; Catalog#80089-R07E; Q00981; Glu 2-Ala 223). UCHL1 specific IgG was purified by Rat UCHL1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-UCHL1 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-UCHL1 Antibody (Rabbit Polyclonal antibody) Images

Rat UCHL1 Immunoprecipitation(IP) 8270

rat UCHL1 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg U87MG Whole Cell Lysate

2 µL anti-rat UCHL1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat UCHL1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Rat UCHL1 Western blot (WB) 13739

Anti-UCHL1 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:25 kDa

(We are unsure as to the identity of these extra bands.)

UCHL1 Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

Full Name
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
References
  • Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92.
  • Choi J., et al., 2004, J. Biol. Chem. 279:13256-64.
  • Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41
  • Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44.
  • Zetterberg, M. et al., 2010, Mol Neurodegener  5 :11.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-UCHL1 Antibody Rabbit Monoclonal WB,ELISA,IP 80089-R012
    Anti-UCHL1 Antibody Rabbit Polyclonal ELISA 80089-RP01
    Anti-UCHL1 Antibody Rabbit Polyclonal ELISA 50690-RP01
    Anti-UCHL1 Antibody Rabbit Monoclonal ELISA 50690-R006
    Anti-UCHL1 Antibody Rabbit Polyclonal WB,ELISA,IP 50690-RP02
    Anti-UCHL1 Antibody Rabbit Monoclonal WB,IP 50690-R011
    Anti-UCHL1 Antibody Rabbit Polyclonal ELISA 11663-RP01
    Anti-UCHL1 Antibody Rabbit Polyclonal WB,ELISA,IP 11663-RP02
    Anti-UCHL1 Antibody Rabbit Monoclonal WB,ELISA,IP 11663-R104
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