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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) CD144/CDH5 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) CD144/CDH5 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10433-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_001795.3
cDNA Size: 2335
Synonym: 7B4; CD144; FLJ17376; CDH5
Source: Human.
cDNA Description: ORF Clone of Homo sapiens cadherin 5, type 2, VE-cadherin (vascular epithelium) DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 384 C/T not causing the amino acid variation and 1550T/C, 1551 C/T resulting in the amino acid 517Ile substitution by Thr.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
1 ATGCAGAGGC TCATGATGCT CCTCGCCACA TCGGGCGCCT GCCTGGGCCT GCTGGCAGTG 61 GCAGCAGTGG CAGCAGCAGG TGCTAACCCT GCCCAACGGG ACACCCACAG CCTGCTGCCC 121 ACCCACCGGC GCCAAAAGAG AGATTGGATT TGGAACCAGA TGCACATTGA TGAAGAGAAA 181 AACACCTCAC TTCCCCATCA TGTAGGCAAG ATCAAGTCAA GCGTGAGTCG CAAGAATGCC 241 AAGTACCTGC TCAAAGGAGA ATATGTGGGC AAGGTCTTCC GGGTCGATGC AGAGACAGGA 301 GACGTGTTCG CCATTGAGAG GCTGGACCGG GAGAATATCT CAGAGTACCA CCTCACTGCT 361 GTCATTGTGG ACAAGGACAC TGGTGAAAAC CTGGAGACTC CTTCCAGCTT CACCATCAAA 421 GTTCATGACG TGAACGACAA CTGGCCTGTG TTCACGCATC GGTTGTTCAA TGCGTCCGTG 481 CCTGAGTCGT CGGCTGTGGG GACCTCAGTC ATCTCTGTGA CAGCAGTGGA TGCAGACGAC 541 CCCACTGTGG GAGACCACGC CTCTGTCATG TACCAAATCC TGAAGGGGAA AGAGTATTTT 601 GCCATCGATA ATTCTGGACG TATTATCACA ATAACGAAAA GCTTGGACCG AGAGAAGCAG 661 GCCAGGTATG AGATCGTGGT GGAAGCGCGA GATGCCCAGG GCCTCCGGGG GGACTCGGGC 721 ACGGCCACCG TGCTGGTCAC TCTGCAAGAC ATCAATGACA ACTTCCCCTT CTTCACCCAG 781 ACCAAGTACA CATTTGTCGT GCCTGAAGAC ACCCGTGTGG GCACCTCTGT GGGCTCTCTG 841 TTTGTTGAGG ACCCAGATGA GCCCCAGAAC CGGATGACCA AGTACAGCAT CTTGCGGGGC 901 GACTACCAGG ACGCTTTCAC CATTGAGACA AACCCCGCCC ACAACGAGGG CATCATCAAG 961 CCCATGAAGC CTCTGGATTA TGAATACATC CAGCAATACA GCTTCATCGT CGAGGCCACA 1021 GACCCCACCA TCGACCTCCG ATACATGAGC CCTCCCGCGG GAAACAGAGC CCAGGTCATT 1081 ATCAACATCA CAGATGTGGA CGAGCCCCCC ATTTTCCAGC AGCCTTTCTA CCACTTCCAG 1141 CTGAAGGAAA ACCAGAAGAA GCCTCTGATT GGCACAGTGC TGGCCATGGA CCCTGATGCG 1201 GCTAGGCATA GCATTGGATA CTCCATCCGC AGGACCAGTG ACAAGGGCCA GTTCTTCCGA 1261 GTCACAAAAA AGGGGGACAT TTACAATGAG AAAGAACTGG ACAGAGAAGT CTACCCCTGG 1321 TATAACCTGA CTGTGGAGGC CAAAGAACTG GATTCCACTG GAACCCCCAC AGGAAAAGAA 1381 TCCATTGTGC AAGTCCACAT TGAAGTTTTG GATGAGAATG ACAATGCCCC GGAGTTTGCC 1441 AAGCCCTACC AGCCCAAAGT GTGTGAGAAC GCTGTCCATG GCCAGCTGGT CCTGCAGATC 1501 TCCGCAATAG ACAAGGACAT AACACCACGA AACGTGAAGT TCAAATTCAC TTTGAATACT 1561 GAGAACAACT TTACCCTCAC GGATAATCAC GATAACACGG CCAACATCAC AGTCAAGTAT 1621 GGGCAGTTTG ACCGGGAGCA TACCAAGGTC CACTTCCTAC CCGTGGTCAT CTCAGACAAT 1681 GGGATGCCAA GTCGCACGGG CACCAGCACG CTGACCGTGG CCGTGTGCAA GTGCAACGAG 1741 CAGGGCGAGT TCACCTTCTG CGAGGATATG GCCGCCCAGG TGGGCGTGAG CATCCAGGCA 1801 GTGGTAGCCA TCTTACTCTG CATCCTCACC ATCACAGTGA TCACCCTGCT CATCTTCCTG 1861 CGGCGGCGGC TCCGGAAGCA GGCCCGCGCG CACGGCAAGA GCGTGCCGGA GATCCACGAG 1921 CAGCTGGTCA CCTACGACGA GGAGGGCGGC GGCGAGATGG ACACCACCAG CTACGATGTG 1981 TCGGTGCTCA ACTCGGTGCG CCGCGGCGGG GCCAAGCCCC CGCGGCCCGC GCTGGACGCC 2041 CGGCCTTCCC TCTATGCGCA GGTGCAGAAG CCACCGAGGC ACGCGCCTGG GGCACACGGA 2101 GGGCCCGGGG AGATGGCAGC CATGATCGAG GTGAAGAAGG ACGAGGCGGA CCACGACGGC 2161 GACGGCCCCC CCTACGACAC GCTGCACATC TACGGCTACG AGGGCTCCGA GTCCATAGCC 2221 GAGTCCCTCA GCTCCCTGGG CACCGACTCA TCCGACTCTG ACGTGGATTA CGACTTCCTT 2281 AACGACTGGG GACCCAGGTT TAAGATGCTG GCTGAGCTGT ACGGCTCGGA CCCCCGGGAG 2341 GAGCTGCTGT ATTAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
