Canine VEGFA 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

VEGFA cDNA Clone Datasheet

VEGFA cDNA Clone Product Information

• Gene Bank Ref.ID: AF133248.1
• cDNA Size: 573
• Gene Synonym: VEGF-A; VPF;
• Source: Canine

VEGFA cDNA Clone Description

• cDNA Description: ORF Clone of Canis lupus vascular endothelial growth factor A DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

VEGFA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VEGFA cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

VEGFA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTTTC TGCTCTCCTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
61       GCCAAGTGGT CCCAGGCTGC GCCTATGGCA GGAGGAGAGC ACAAACCCCA CGAAGTGGTG
121      AAGTTCATGG ACGTCTACCA GCGCAGCTAC TGCCGTCCCA TTGAGACCCT GGTGGACATC
181      TTCCAGGAGT ACCCTGACGA GATCGAGTAC ATCTTCAAGC CATCCTGCGT GCCCCTGATG
241      CGGTGTGGGG GCTGCTGTAA TGATGAGGGC CTAGAGTGCG TGCCCACTGA GGAGTTCAAC
301      ATCACCATGC AGATTATGCG GATCAAACCT CATCAAGGCC AGCACATAGG GGAGATGAGT
361      TTCCTGCAGC ATAGCAAATG TGAATGCAGA CCAAAGAAAG ATAGAGCAAG GCAAGAAAAT
421      CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
481      AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
541      CGTACTTGCA GATGTGACAA GCCCAGGCGG TGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).