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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) Vitronectin 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) Vitronectin 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10424-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_000638.3
cDNA Size: 1437
Synonym: VN, V75, VNT, VTN
Source: Human.
cDNA Description: ORF Clone of Homo sapiens vitronectin DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1199C/T resulting in the amino acid 400Thr substitution by Met.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
1 ATGGCACCCC TGAGACCCCT TCTCATACTG GCCCTGCTGG CATGGGTTGC TCTGGCTGAC 61 CAAGAGTCAT GCAAGGGCCG CTGCACTGAG GGCTTCAACG TGGACAAGAA GTGCCAGTGT 121 GACGAGCTCT GCTCTTACTA CCAGAGCTGC TGCACAGACT ATACGGCTGA GTGCAAGCCC 181 CAAGTGACTC GCGGGGATGT GTTCACTATG CCGGAGGATG AGTACACGGT CTATGACGAT 241 GGCGAGGAGA AAAACAATGC CACTGTCCAT GAACAGGTGG GGGGCCCCTC CCTGACCTCT 301 GACCTCCAGG CCCAGTCCAA AGGGAATCCT GAGCAGACAC CTGTTCTGAA ACCTGAGGAA 361 GAGGCCCCTG CGCCTGAGGT GGGCGCCTCT AAGCCTGAGG GGATAGACTC AAGGCCTGAG 421 ACCCTTCATC CAGGGAGACC TCAGCCCCCA GCAGAGGAGG AGCTGTGCAG TGGGAAGCCC 481 TTCGACGCCT TCACCGACCT CAAGAACGGT TCCCTCTTTG CCTTCCGAGG GCAGTACTGC 541 TATGAACTGG ACGAAAAGGC AGTGAGGCCT GGGTACCCCA AGCTCATCCG AGATGTCTGG 601 GGCATCGAGG GCCCCATCGA TGCCGCCTTC ACCCGCATCA ACTGTCAGGG GAAGACCTAC 661 CTCTTCAAGG GTAGTCAGTA CTGGCGCTTT GAGGATGGTG TCCTGGACCC TGATTACCCC 721 CGAAATATCT CTGACGGCTT CGATGGCATC CCGGACAACG TGGATGCAGC CTTGGCCCTC 781 CCTGCCCATA GCTACAGTGG CCGGGAGCGG GTCTACTTCT TCAAGGGGAA ACAGTACTGG 841 GAGTACCAGT TCCAGCACCA GCCCAGTCAG GAGGAGTGTG AAGGCAGCTC CCTGTCGGCT 901 GTGTTTGAAC ACTTTGCCAT GATGCAGCGG GACAGCTGGG AGGACATCTT CGAGCTTCTC 961 TTCTGGGGCA GAACCTCTGC TGGTACCAGA CAGCCCCAGT TCATTAGCCG GGACTGGCAC 1021 GGTGTGCCAG GGCAAGTGGA CGCAGCCATG GCTGGCCGCA TCTACATCTC AGGCATGGCA 1081 CCCCGCCCCT CCTTGGCCAA GAAACAAAGG TTTAGGCATC GCAACCGCAA AGGCTACCGT 1141 TCACAACGAG GCCACAGCCG TGGCCGCAAC CAGAACTCCC GCCGGCCATC CCGCGCCATG 1201 TGGCTGTCCT TGTTCTCCAG TGAGGAGAGC AACTTGGGAG CCAACAACTA TGATGACTAC 1261 AGGATGGACT GGCTTGTGCC TGCCACCTGT GAACCCATCC AGAGTGTCTT CTTCTTCTCT 1321 GGAGACAAGT ACTACCGAGT CAATCTTCGC ACACGGCGAG TGGACACTGT GGACCCTCCC 1381 TACCCACGCT CCATCGCTCA GTACTGGCTG GGCTGCCCAG CTCCTGGCCA TCTGTAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
