휴먼(Human) Wnt-7a 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10425-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_004625.3

cDNA Size:   1050

Synonym:  WNT7A

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens wingless-type MMTV integration site family, member 7A DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAACCGGA AAGCGCGGCG CTGCCTGGGC CACCTCTTTC TCAGCCTGGG CATGGTCTAC
61       CTCCGGATCG GTGGCTTCTC CTCAGTGGTA GCTCTGGGCG CAAGCATCAT CTGTAACAAG
121      ATCCCAGGCC TGGCTCCCAG ACAGCGGGCG ATCTGCCAGA GCCGGCCCGA CGCCATCATC
181      GTCATAGGAG AAGGCTCACA AATGGGCCTG GACGAGTGTC AGTTTCAGTT CCGCAATGGC
241      CGCTGGAACT GCTCTGCACT GGGAGAGCGC ACCGTCTTCG GGAAGGAGCT CAAAGTGGGG
301      AGCCGGGAGG CTGCGTTCAC CTACGCCATC ATTGCCGCCG GCGTGGCCCA CGCCATCACA
361      GCTGCCTGTA CCCAGGGCAA CCTGAGCGAC TGTGGCTGCG ACAAAGAGAA GCAAGGCCAG
421      TACCACCGGG ACGAGGGCTG GAAGTGGGGT GGCTGCTCTG CCGACATCCG CTACGGCATC
481      GGCTTCGCCA AGGTCTTTGT GGATGCCCGG GAGATCAAGC AGAATGCCCG GACTCTCATG
541      AACTTGCACA ACAACGAGGC AGGCCGAAAG ATCCTGGAGG AGAACATGAA GCTGGAATGT
601      AAGTGCCACG GCGTGTCAGG CTCGTGCACC ACCAAGACGT GCTGGACCAC ACTGCCACAG
661      TTTCGGGAGC TGGGCTACGT GCTCAAGGAC AAGTACAACG AGGCCGTTCA CGTGGAGCCT
721      GTGCGTGCCA GCCGCAACAA GCGGCCCACC TTCCTGAAGA TCAAGAAGCC ACTGTCGTAC
781      CGCAAGCCCA TGGACACGGA CCTGGTGTAC ATCGAGAAGT CGCCCAACTA CTGCGAGGAG
841      GACCCGGTGA CCGGCAGTGT GGGCACCCAG GGCCGCGCCT GCAACAAGAC GGCTCCCCAG
901      GCCAGCGGCT GTGACCTCAT GTGCTGTGGG CGTGGCTACA ACACCCACCA GTACGCCCGC
961      GTGTGGCAGT GCAACTGTAA GTTCCACTGG TGCTGCTATG TCAAGTGCAA CACGTGCAGC
1021     GAGCGCACGG AGATGTACAC GTGCAAGTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) Wnt-7a 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)