Ab Production Service Q&A

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Antibody Production Service Q&A

Q: What is included in one-stop-shop antibody production service package?
A: One-stop-shop antibody production service includes gene synthesis, vector construction, plasmid production, expression, purification and QC.

Q: What information or material do I need to provide to Sino Biological and what deliverables do I get?
A: Clients could either provide gene sequences by e-mail or plasmids as starting materials. The deliverables could be purified high-quality antibody, antibody genes, and COAs. Please refer to the request form for required information.

Q:I want to send plasmids to Sino Biological. How do I do?
A: Please prepare plasmids according to the following protocol for keeping plasmid in FTA elute card (Whatman Cat. No.: WB120410, WB120401, WB120461) at room temperature.
HANDLING: Always wear gloves to avoid contamination of FTA Elute Cards.
1). Label the FTA Elute Card with the appropriate sample identification. Use one card for each biological sample.
2). Pipet the biological sample (40μl per 11 mm circle) onto the card in a concentric circular motion within the printed circle area slowly.
3). Samples applied to FTA Elute Cards are ready for immediate room temperature storage. Note: If samples are to be processed shortly after application on the FTA Elute Card, allow the sample to dry completely for at least 3 hours at room temperature prior to punching. To reduce drying time, spotted FTA Elute cards can be placed at 80° C for 15-20 minutes.

Q: What are the milestones and what is the timeline?
A: The milestones are gene synthesis (2-3 weeks), vector construction (1 week), plasmid production (3 days), and antibody production (2-4 weeks; including expression, purification and QC).

Q: Do I get timely update?
A: Client can either choose to receive updates at each milestone or just to receive notice when there is any problem which could cause delays. In most cases, you can just send us the request and in a couple of weeks, you will get an e-mail from our project manager that the project is completed and the product is going to be delivered to you shortly. If you prefer to get frequent project update, we can also accommodate that to meet your requirement.

Q: What is the payment term?
A: 50% upfront payment is required before project initiation. After completion of the project, the rest should be paid within 30days upon product delivery.

Q: Do I get refund if the project fails?
A: If antibody expression yield is too low or prone to aggregation, a revised price quotation will be provided after the initial assessment of productivity. Client has the option to reject the revised pricing and terminate the project with a minimum evaluation fee of $500, plus cost for gene synthesis and vector construction. If the total charge is greater than your upfront payment, we will send you back a refund within 30-days. We have extremly high success rate for antibody production , so this rarely happens.

Q: What purity and concentration of the final product does Sino Biological usually provide?
A: The final purity and concentration differs from product to product according to client's specific requirements. We can meet very high antibody quality standard equivalent to GLP and GMP standards.

Q:Can I have customized concentration and formulation?
A: Yes. Client could require customized final concentration and formulation according to downstream work. New proposals will be provided if there are any problems with the required concentration and formulation.

Q : Can I get the antibody expression vector as part of deliverable?
A: The antibody expression vector used in Sino Biological is optimized and confidential. A commercial expression vector could be provided as part of deliverables according to client's requirement.

Q: How to Submit a Request for Antibody Production Service?
A: If you are interested in using our antibody production services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CRO-service@sinobiological.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.

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