|Send Mab Seq||Receive Purified Mab||Make Payment Upon Satisfaction||Inquire Now!
|No Upfront Payment!||No Risk to You!||You Order,We Deliver!|
|Host Cells:||HEK293 or CHO cell (please specify, cost for production in CHO cell is higher)
We provide antibody Fab production in E. coli. expression system as well,
If you prefer E. coli. system, please specify in your request.
|Production:||Recombinant technology, transient transfection, Serum-free cell culture
|Purification:||His-tag or FLAG-tag Affinity chromatography column purification
Protein A or G affinity purification maybe feasible for some Fabs without a His-tag/FLAG tag
|Timeline:||5-8 weeks total from receiving gene sequence to antibody delivery
- 1-2 weeks for gene synthesis
- 1-2 weeks for vector construction and plasmid production
- 3-4 weeks for transient transfection, antibody purification, QC testing
|Deliverable:||Purified antibody Fab (quantities to be specified by customer)
|Quality Specification:||as specified by cutomer (cost may be adjusted based on quality spec)
|Antibody Types:||human, mouse, rat, rabbit, canine, rhesus IgG antibodies
|Applications:||drug discovery: antibody antigen co-crystalization, antibody binding eptiope|
|Quotation:||on-line request form or e-mail request to CROemail@example.com|
• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas
|Cutting-Edge Technology||Unlimited Capacity||Unbelievable Cost-Saving|
Sino Biological's has developed transient transfection technology in HEK293 cells for antibody Fab expression and production, and has generated excellent quality of Fab fragment for antigen-Fab co-crystallization. The recombinant antibody Fab fragment protein produced by our transient transfection in mammalian cells is of superior quality than antibody fragment protein generated from enzyme cleavage of full antibodies. If customers prefer, we can also use our E. coli. expression system for antibody Fab production.
Because many antibody Fab fragment proteins do not bind well to protein A, protein G, or protein L affinity resin, we typically add a poly-histidine tag at the C-terminus of the antibody heave chain for purification. A protease cleavage site can be designed between the His-tag and the Fab to allow tag-removal after purification if needed.
You Pay on Deliverable, Risk-Free:
For customers that have set up a contract service account with us, we typically do not require an upfront payment ...... <Learn More>