FGF2 Protein, Human, Recombinant

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FGF2 Protein, Human, Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.02-0.1 ng/ml.
Protein Construction
A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human bFGF consists of 147 amino acids and predicts a molecular mass of 16.5 kDa.
Formulation
Lyophilized from sterile 20mM Tris, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF2 Protein, Human, Recombinant: Images

FGF2 Protein, Human, Recombinant: Alternative Names

BFGF Protein, Human; FGF-2 Protein, Human; FGFB Protein, Human; HBGF-2 Protein, Human

FGF2 Background Information

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

bFGF/FGF2 Protein & Antibody Products

Full Name
fibroblast growth factor 2 (basic)
References
  • Takayama S,et al. (2001) Periodontal regeneration by FGF-2 (bFGF) in primate models. J Dent Res. 80(12): 2075-9.
  • Niu YJ, et al. (2004) Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chin Med J (Engl). 117(2): 252-7.
  • Zhang Y,et al. (2004) Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chin Med J (Engl). 117(4): 601-3.
  • Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System
    Author
    Quan, L;Chen, Y;Song, J;Yan, Q;Zhang, Q;Lai, S;Fan, N;Xin, J;Zou, Q;Lai, L;
    Year
    2014
    Journal
    PLoS ONE
    Application
    culture
  • A lab-centred approach for initiating early stage regeneration of dental pulp using specialised alginate biomimetic microenvironments
    Author
    Bhoj, M;
    Year
    2015
    Journal
    HKU Theses Online (HKUTO)
  • Expression of functional recombinant human fibroblast growth factor 8b and its protective effects on MPP(+)-lesioned PC12 cells
    Author
    Chen, N;Ma, J;Zhao, Y;Wu, M;Yang, H;Gong, W;Chao, J;Li, X;
    Year
    2015
    Journal
    Appl. Microbiol. Biotechnol.
    Application
    cell-based
  • Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion
    Author
    Yang, X;Steinberg, F;Zhuang, L;Bessey, R;Trueb, B;
    Year
    2016
    Journal
    Int. J. Mol. Med.
    Application
    cell-based
  • PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs
    Author
    Shan, Y;Liang, Z;Xing, Q;Zhang, T;Wang, B;Tian, S;Huang, W;Zhang, Y;Yao, J;Zhu, Y;Huang, K;Liu, Y;Wang, X;Chen, Q;Zhang, J;Shang, B;Li, S;Shi, X;Liao, B;Zhang, C;Lai, K;Zhong, X;Shu, X;Wang, J;Yao, H;Chen, J;Pei, D;Pan, G;
    Year
    2017
    Journal
    Nat Commun
  • Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory Pattern
    Author
    Wang, J;Li, J;Gu, Y;Xia, Q;Song, W;Zhang, X;Yang, Y;Wang, W;Li, H;Zou, K;
    Year
    2018
    Journal
    bioRxiv
    Application
    cell-based
  • In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells
    Author
    Zhao, Y;Ye, S;Liang, D;Wang, P;Fu, J;Ma, Q;Kong, R;Shi, L;Gong, X;Chen, W;Ding, W;Yang, W;Zhu, Z;Chen, H;Sun, X;Zhu, J;Li, Z;Wang, Y;
    Year
    2018
    Journal
    Stem Cell Reports
    Application
    cell-based (hESC/iPS)
  • Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination
    Author
    Liu, Z;Yan, M;Liang, Y;Liu, M;Zhang, K;Shao, D;Jiang, R;Li, L;Wang, C;Nussenzveig, DR;Zhang, K;Chen, S;Zhong, C;Mo, W;Fontoura, BMA;Zhang, L;
    Year
    2019
    Journal
    Neuron
    Application
    cell-based
  • A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism
    Author
    Ou, Z;Ma, Y;Sun, Y;Zheng, G;Wang, S;Xing, R;Chen, X;Han, Y;Wang, J;Lu, QR;Zhao, TJ;Chen, Y;
    Year
    2019
    Journal
    Cell Rep
    Application
    cell-based
  • Characterization of porcine extraembryonic endoderm cells
    Author
    Shen, QY;Yu, S;Zhang, Y;Zhou, Z;Zhu, ZS;Pan, Q;Lv, S;Niu, HM;Li, N;Peng, S;Liao, MZ;Wang, HY;Lei, AM;Miao, YL;Liu, ZH;Hua, JL;
    Year
    2019
    Journal
    Cell Prolif.
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