CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb

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CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human CD32A
Has cross-reactivity with
Human CD32b
No cross-reactivity with Human CD16a, Human CD16b and Human cell lysate (293 cell line) in ELISA
Immunogen
Recombinant Human CD32a protein (Catalog#10374-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD32a extracellular domain (rh CD32a; Catalog#10374-H08H; Met 1-Ile 218; AAA35827.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #4B7C6A2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human CD32 expression on human whole blood lymphocytes (left panel), monocytes (middle panel) and granulocytes (right panel). Cells were stained with purified anti-Human CD32, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes, monocytes and granulocytes.

CD32/FCGR2/Fc gamma RII Antibody, Mouse MAb: Alternative Names

Anti-CD32 Antibody; Anti-CD32A Antibody; Anti-CDw32 Antibody; Anti-Fc gamma RIIA Antibody; Anti-FCG2 Antibody; Anti-FcGR Antibody; Anti-FCGR2 Antibody; Anti-FCGR2A1 Antibody; Anti-IGFR2 Antibody
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