Anti-EGFR Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
Human EGFR No cross-reactivity in ELISA with Human ErbB2 Human ErbB3 Human ErbB4
Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #08
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
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