Anti-EpCAM Antibody

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Anti-EpCAM Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-EpCAM Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human EpCAM
No cross-reactivity in ELISA with
Human CD146 / MCAM
Human CD171 / L1CAM
Human OBCAM / OPCML
Human TROP2 / TACSTD2
Immunogen
Recombinant Human EpCAM protein (Catalog#10694-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #4A8H7D12
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EpCAM Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EpCAM Antibody (Mouse Monoclonal antibody) Images

Anti-EPCAM mouse monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: HCT116 Whole Cell Lysate

Lane D: COLO205 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:40 kDa

EPCAM was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-EPCAM mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-EPCAM mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Anti-EpCAM Antibody: Alternative Names

Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP40 Antibody; Anti-ESA Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-M4S1 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP-1 Antibody; Anti-TROP1 Antibody

EpCAM Background Information

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
Full Name
epithelial cell adhesion molecule
References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.

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