Factor VII Matched ELISA Antibody Pair Set,Mouse

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Factor VII Matched ELISA Antibody Pair Set,Mouse General Information

Product name
Factor VII Matched ELISA Antibody Pair Set,Mouse
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Mouse Factor VII
Assay range
23.44-1500 pg/mL
Materials provided
1. Capture Antibody 0.2 mg/mL of rabbit anti-Mouse Factor VII/F7 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50034-R003)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse Factor VII/F7 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50034-R121)
3. Standard Each vial contains 28.5 ng of recombinant Mouse Factor VII/F7. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1600 pg/mL is recommended.
Product overview
This Factor VII Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Factor VII . It contains Mouse Factor VII capture antibody, Mouse Factor VII detector antibody (HRP) and a highly purified CHO Stable-expressed recombinant Mouse Factor VII protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Factor VII Matched ELISA Antibody Pair Set,Mouse Images

Factor VII Matched ELISA Antibody Pair Set,Mouse: Alternative Names

AI132620 Matched ELISA Antibody Pair Set, Mouse; Cf7 Matched ELISA Antibody Pair Set, Mouse; FVII Matched ELISA Antibody Pair Set, Mouse

Factor VII Background Information

Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class, and Factor VII (FVII) deficiency is the most frequent among rare congenital bleeding disorders. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Recombinant activated factor VII (rFVIIa) is a haemostatic agent, which was originally developed for the treatment of haemophilia patients with inhibitors against factor FVIII or FIX. FVIIa binds specifically to endothelial protein C receptor (EPCR), a known cellular receptor for protein C and activated protein C, on the endothelium. rFVIIa is a novel hemostatic agent, originally developed for the treatment of hemorrhage in hemophiliacs with inhibitors, which has been successfully used recently in an increasing number of nonhemophilic bleeding conditions.
Full Name
coagulation factor VII (serum prothrombin conversion accelerator)
References
  • Franchini M, et al. (2007) Potential role of recombinant activated factor VII for the treatment of severe bleeding associated with disseminated intravascular coagulation: a systematic review. Blood Coagul Fibrinolysis. 18(7): 589-93.
  • Lapecorella M, et al. (2008) Factor VII deficiency: defining the clinical picture and optimizing therapeutic options. Haemophilia. 14(6): 1170-5.
  • Grottke O, et al. (2010) Activated recombinant factor VII (rFVIIa). Best Pract Res Clin Anaesthesiol. 24(1): 95-106.
  • The production of coagulation factor VII by adipocytes is enhanced by tumor necrosis factor-α or isoproterenol
    Author
    Takahashi, N;Yoshizaki, T;Hiranaka, N;Kumano, O;Suzuki, T;Akanuma, M;Yui, T;Kanazawa, K;Yoshida, M;Naito, S;Fujiya, M;Kohgo, Y;Ieko, M;
    Year
    2014
    Journal
    Int J Obes (Lond)
    Application
    ELISA
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