15-PGDH ELISA Kit, Human

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15-PGDH ELISA Kit, Human: General Information

Product name
15-PGDH ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Lysate
Sensitivity
19.2 pg/mL
Assay range
54.69-3500 pg/mL
Specificity
Recognizes both recombinant and natural Human 15-PGDH
Interference
Preparations of huaman APP at 20 ng/mL in a mid-range recombinant human HPGD control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 453.80 829.92 1,712.26
SD 14.95 24.66 54.59
CV(%) 3.3% 3.0% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 486.65 954.63 1,965.70
SD 28.54 109.99 219.87
CV(%) 5.9% 11.5% 11.2%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This 15-PGDH ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human 15-PGDH protein in Lysate . It contains recombinant Human 15-PGDH, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

15-PGDH ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

15-PGDH ELISA Kit, Human: Alternative Names

15-PGDH ELISA Kit, Human; PGDH ELISA Kit, Human; PGDH1 ELISA Kit, Human; PHOAR1 ELISA Kit, Human; SDR36C1 ELISA Kit, Human

15-PGDH Background Information

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor inmany species and regulate uterine contractility and cervicaldilatation. Therefore, the regulation of prostaglandin outputby PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activitypatterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR), cranioosteoarthropathy (COA),and isolated congenital nail clubbing.
Full Name
hydroxyprostaglandin dehydrogenase 15-(NAD)
References
  • Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33.
  • McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41.
  • Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73.
  • Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
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