휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone in cloning vector

    데이터시트리뷰관련제품프로토콜
    휴먼 MAPK14 cDNA 클론 제품 정보
    Gene_bank_ref_id:NM_139012.1
    cDNA 크기:1083bp
    cDNA 설명:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 14 transcript variant 2.
    유전자 동의어:MAPK14, RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA
    :Human
    벡터:pMD18-T Simple Vector
    Plasmid:pMD-P38αMAPK
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:M13-47 and RV-M
    ( We provide with MAPK14 qPCR primers for gene expression analysis, HP100167 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone in cloning vector on other vectors
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG10081-ACG265260
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG10081-ACR265260
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG10081-ANG265260
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG10081-ANR265260
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10081-CF229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-His 태그HG10081-CH229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG10081-CM229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA 태그HG10081-CY229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG10081-NF229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-His 태그HG10081-NH229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG10081-NM229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-HA 태그HG10081-NY229890
    휴먼 p38 alpha/MAPK14 transcript variant 2 Gene ORF cDNA clone expression plasmidHG10081-UT229890
     발현 벡터에 대해 자세히 알아보기
    Product nameProduct name
    연구배경

    MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK14 can be detected in brain, heart, placenta, pancreas and skeletal muscle and it is expressed to a lesser extent in lung, liver and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. In respond to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide, MAPK14 phosphorylates a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example IL-6. It may play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.

    참고자료
  • Luo X, et al. (2011) Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia. Zhonghua Fu Chan Ke Za Zhi. 46(1):36-40.
  • Park CH, et al. (2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci. 64(2):134-41.
  • Lee JY, et al. (2011) Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PLoS One. 6(8):e23756.
  • Riis JL, et al. (2011) CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways. Cytokine. 56(3):699-707.
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    Cat No: HG10081-M
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