Stable Cell Line Development Service (HEK293 / CHO)

Stable Cell Line Development Service Introduction

Stable cell line is widely used in various areas, such as recombinant protein and antibody production, testing and analysis, gene editing, functional study and so on. Engaged in protein expression study for years and possessing abundant stable cell line development experience, Sino Biological can effectively increase stable cell line productivity and improve experimental results. Integrating all the experimental steps, we provide one-stop service from gene sequence to deliverable stable cell line.

Stable Cell Line Development Service Items

CHO stable cell line development

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks   Please fill in the form and send it to
cro-service@sinobiological.com

Vector construction
• Cloning cDNA into expression vector
• Plasmid sequencing
• Large-scale plasmid preparation
2-3 weeks • Sequencing report

Transient expression evaluation
• Transient expression
• Expression level detection by ELISA or WB
2 weeks • Evaluation report

Stable cell line development
• Stable transfection and screening
• High expression clone screening and stabllity screening
• Cell bank construction
12-20 weeks • Expression report
• 2-3 stable clones (optional)

Large-scale expression and purification (optional)
  2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

HEK293 stable cell line development

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks   Please fill in the form and send it to
cro-service@sinobiological.com

Vector construction
• cloning cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
2-3 weeks • Sequencing report

Transient expression evaluation
• Transient expression
• Expression level detection by ELISA or WB
2 weeks • Evaluation report

Stable cell line construction
• stable transfection and screening
• Identification (qPCR and / or FACS)
• Subcloning
• Identification (qPCR and / or FACS)
• cell bank construction
10-12 weeks • Identification report
• 2 tube cells(1×10^6 cells / tube)

Large-scale expression and purification (optional)
  2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

Stable Cell Line Development Service Procedures

Experimental requirements and price communication   Contract signing and payment in advance   Transient expression evaluation (CHO cell)   Stable cell line construction and selection   Stable cell line providing   Large-scale protein expression and purification (optional)   Product delivery and payment in the rest

Contact Us

Tel
+86-10-58628216; +86-10-58628223
Email
cro-service@sinobiological.com