VSIG4 ELISA Kit, Human

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VSIG4 ELISA Kit, Human: General Information

Product name
VSIG4 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Lysate
Sensitivity
12.63 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Human VSIG4
Interference
Preparations of APP、FLT4、NEF and GK at 25 ng/mL in a mid-range recombinant human VSIG4 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.22 0.40 0.84
SD 0.02 0.04 0.07
CV(%) 8.2% 10.1% 8.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.25 0.46 0.93
SD 0.02 0.05 0.08
CV(%) 7.8% 10.6% 8.1%
Recovery
The recovery of Human VSIG4 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
85
Range
81-88%
Linearity
Supernatant
  Recovery of detected
1:2 83%
1:4 82%
1:8 81%
1:16 88%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This VSIG4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VSIG4 protein in Lysate . It contains recombinant Human VSIG4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

VSIG4 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

VSIG4 ELISA Kit, Human: Alternative Names

CRIg ELISA Kit, Human; Z39IG ELISA Kit, Human

VSIG4 Background Information

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type Ig domain and a truncated C-type Ig domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, an V-type Ig domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type Ig domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type Ig domains of rat and human VSIG4, respectively.
Full Name
V-set and immunoglobulin domain containing 4
References
  • Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826.
  • Helmy, K. et al., 2006, Cell. 124:915-927.
  • Wiesmann, C. et al., 2006, Nature. 444:217-220.
  • Zang,X. et al., 2006, J Clin Invest. 116: 2590-2593.
  • Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325.
  • He,JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
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