Anti-4-1BBL Antibody

Cat: 50067-R014

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Anti-4-1BBL Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-4-1BBL Antibody
Applications
ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Mouse
Specificity
Mouse 4-1BBL
Immunogen
Recombinant Mouse TNFSF9 protein (Catalog#50067-M07H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFSF9 / 4-1BBL (rM TNFSF9; Catalog#50067-M07H; Arg 104-Glu 309; NP_033430.1).
Source
Monoclonal Rabbit IgG Clone #014
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-4-1BBL Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-4-1BBL Antibody (Rabbit Monoclonal antibody) Images

Mouse CD137L/4-1BBL/TNFSF9 Immunohistochemistry(IHC) 5472
Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Mouse CD137L/4-1BBL/TNFSF9 Flow Cytometry (FC) 14788
Flow cytometric analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-4-1BBL Antibody Alternative Names

Anti-4-1BB-L Antibody;Anti-4-1BBL Antibody;Anti-AI848817 Antibody;Anti-Cd137l Antibody;Anti-Ly63l Antibody

4-1BBL Background Information

4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Full Name
tumor necrosis factor (ligand) superfamily, member 9
References
  • Cheung CT, (2007) Neutralizing anti-4-1BBL treatment improves cardiac function in viral myocarditis. Lab Invest. 87(7): 651-61.
  • Wang C, et al. (2009) Immune regulation by 4-1BB and 4-1BBL: complexities and challenges. Immunol Rev. 229(1): 192-215.
  • Habib-Agahi M, et al. (2009) 4-1BBL costimulation retrieves CD28 expression in activated T cells. Cell Immunol. 256(1-2): 39-46.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-4-1BBL Antibody Rabbit Polyclonal ELISA 80166-T16
    Anti-4-1BBL Antibody Rabbit Polyclonal ELISA 80166-RP01
    Anti-4-1BBL Antibody Rabbit Polyclonal ELISA 50067-RP02
    Anti-4-1BBL Antibody (APC) Rabbit Monoclonal FCM 50067-R014-A
    Anti-4-1BBL Antibody (FITC) Rabbit Monoclonal FCM 50067-R014-F
    Anti-4-1BBL Antibody (PerCP) Rabbit Monoclonal FCM 50067-R014-C
    Anti-4-1BBL Antibody Rabbit Polyclonal ELISA 50067-RP01
    Anti-4-1BBL Antibody (PE) Rabbit Monoclonal FCM 50067-R014-P
    Anti-4-1BBL Antibody Rabbit Polyclonal ELISA 50067-T16
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