Anti-Endoglin / CD105 Antibody

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Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Endoglin / CD105 Antibody
Validated applications
ELISA,IHC-P,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Endoglin / CD105
Immunogen
Recombinant Human Endoglin/CD105 Protein (Catalog#10149-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Endoglin/CD105 (rh Endoglin/CD105 ; Catalog#10149-H08H; NP_001108225.1; Met1-Gly586). Endoglin/CD105 specific IgG was purified by Human Endoglin/CD105 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IHC-P 1:2000-1:10000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human CD105 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CD105 in human renal carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CD105 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of CD105 in U251MG cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CD105 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.

Anti-Endoglin / CD105 Antibody: Alternative Names

Anti-END Antibody; Anti-HHT1 Antibody; Anti-ORW1 Antibody

Endoglin / CD105 Background Information

Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
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