Anti-HA tag Antibody

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Anti-HA tag Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-HA tag Antibody
Validated applications
IP
Specificity
HA tag
Immunogen
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Clone #15
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HA tag Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
IP 4-8 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HA tag Antibody (Mouse Monoclonal antibody) Images

HA tag was immunoprecipitated using:

Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HA tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

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