Anti-ICAM-1 Antibody

Price:
Size:
Number:

Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-ICAM-1 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Rat
Specificity
Rat ICAM-1
Immunogen
Recombinant Rat ICAM-1/CD54 Protein (Catalog#80022-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat ICAM-1/CD54 (rR ICAM-1/CD54; Catalog#80022-R08H; Q00238-1; Met1-Thr493). ICAM-1/CD54 specific IgG was purified by affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:10000
IP 5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) Images

Anti-ICAM1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Rat Spleen tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:65 kDa

Immunochemical staining of rat ICAM1 in rat kidney with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of rat ICAM1 in rat liver with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.

ratICAM1 was immunoprecipitated using:

Lane A:0.5 mg NIH-3T3 Whole Cell Lysate

2 µL anti-ratICAM1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ratICAM1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 60 kDa

Observed band size :55 kDa

ICAM-1 Background Information

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
Full Name
intercellular adhesion molecule 1
Research Areas
References
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait