Anti-IFNAR1 Antibody

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Anti-IFNAR1 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-IFNAR1 Antibody
Validated applications
ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human IFNAR1
Immunogen
Recombinant Human IFNAR1 Protein (Catalog#13222-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human IFNAR1 (rh IFNAR1; Catalog#13222-H08H; P17181-1; Met1-Lys436). IFNAR1 specific IgG was purified by Human IFNAR1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IFNAR1 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IP IP:1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IFNAR1 Antibody (Rabbit Polyclonal antibody) Images

IFNAR1 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

4 µL anti-IFNAR1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-IFNAR1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 64 kDa

Observed band size :130 kDa

Anti-IFNAR1 Antibody: Alternative Names

Anti-AVP Antibody; Anti-IFN-alpha-REC Antibody; Anti-IFNAR Antibody; Anti-IFNBR Antibody; Anti-IFRC Antibody

IFNAR1 Background Information

Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
Full Name
interferon (alpha, beta and omega) receptor 1
References
  • Yan H, et al. (1996) Phosphorylated interferon-alpha receptor 1 subunit (IFNaR1) acts as a docking site for the latent form of the 113 kDa STAT2 protein. EMBO J. 15(5): 1064-74.
  • Richter MF, et al. (1998) Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. J Biol Chem. 273(38): 24723-9.
  • Abramovich C, et al. (1997) A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor. EMBO J. 16(2): 260-6.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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