Anti-CD55 / DAF Antibody (APC)

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Anti-CD55 / DAF Antibody (APC) (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD55 / DAF Antibody (APC)
Validated applications
FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD55 / DAF
Immunogen
Recombinant Mouse CD55 / DAF protein (Catalog#50468-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD55 / DAF (rM CD55 / DAF; Catalog#50468-M08H; Q61475; Met1-Thr361) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Source
Monoclonal Rabbit IgG Clone #076
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
APC
Concentration
5 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-CD55 / DAF Antibody (APC) (Rabbit Monoclonal antibody) Images

Flow cytometric analysis of Mouse CD55 expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse CD55. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-CD55 / DAF Antibody (APC): Alternative Names

Anti-Daf Antibody; Anti-Daf-GPI Antibody; Anti-Daf1 Antibody; Anti-GPI-DAF Antibody

CD55 / DAF Background Information

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 3 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 7-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
Full Name
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
References
  • Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.
  • Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.
  • Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.
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