Anti-Carboxypeptidase A Antibody

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Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Carboxypeptidase A Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human Carboxypeptidase A
Immunogen
Recombinant Human Carboxypeptidase A1 / CPA1 protein (Catalog#10504-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Carboxypeptidase A1 / CPA1 (rh Carboxypeptidase A1 / CPA1; Catalog#10504-H08H; NP_001859.1; Met 1-Tyr 419). Carboxypeptidase A1 / CPA1 specific IgG was purified by Human Carboxypeptidase A1 / CPA1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human CPA1 in human pancreas with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

CPA1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-CPA1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CPA1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 50 kDa

Anti-CPA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 60 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:47 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Carboxypeptidase A Antibody: Alternative Names

Anti-CPA Antibody

Carboxypeptidase A Background Information

Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
Full Name
carboxypeptidase A1 (pancreatic)
References
  • Catasus, L. et al., 1992, Biochem. J. 287: 299-303.
  • Moulard, M. et al., 1990, FEBS. Lett. 261: 179-183.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
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