EGFR Protein, Mouse, Recombinant (His Tag): Product Information
> 95 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc (Cat:10605-H01H), The EC50 of human EGF-Fc (Cat:10605-H01H) is 60-90 ng/mL. 3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc (Cat:50482-M01H), The EC50 of mouse EGF-Fc (Cat:50482-M01H) is 70-100 ng/mL.
A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
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