Anti-EGFR Antibody

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Anti-EGFR Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-EGFR Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse EGFR
Immunogen
Recombinant Mouse EGFR / HER1 / ErbB1 Protein (Catalog#51091-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse EGFR / HER1 / ErbB1 (rM EGFR / HER1 / ErbB1; Catalog#51091-M08H; Q01279; Met1-Ser647). EGFR / HER1 / ErbB1 specific IgG was purified by Mouse EGFR / HER1 / ErbB1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Polyclonal antibody) Images

Mouse EGFR was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

1 µL anti-Mouse EGFR rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse EGFR rabbit polyclonal antibody,at 1:350 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 170 kDa

Observed band size: 170 kDa

Anti-EGFR rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:170 kDa

Observed band size:170 kDa

(We are unsure as to the identity of these extra bands.)

Anti-EGFR Antibody: Alternative Names

Anti-9030024J15Rik Antibody; Anti-AI552599 Antibody; Anti-Erbb Antibody; Anti-Errb1 Antibody; Anti-Errp Antibody; Anti-wa-2 Antibody; Anti-wa2 Antibody; Anti-Wa5 Antibody

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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