EGFR Protein, Rat, Recombinant (His Tag)

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EGFR Protein, Rat, Recombinant (His Tag): Product Information

Purity
> 98 % as determined by SDS-PAGE
Activity
1. Measured by its ability to bind recombinant human EGF-Fc (Cat:10605-H01H) in a functional ELISA.
2. Measured by its ability to bind recombinant mouse EGF-Fc (Cat:50482-M01H) in a functional ELISA.
Protein Construction
A DNA sequence encoding the rat EGFR (E7CXR8) (Met1-Ser646) was expressed, fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Rat
Predicted N Terminal
Leu 25
Molecule Mass
The recombinant rat EGFR comprises 633 amino acids and predicts a molecular mass of 70.7 kDa. The apparent molecular mass of the recombinant protein is approximately 93-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Rat, Recombinant (His Tag): Images

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Targeting neratinib-induced diarrhea with budesonide and colesevelam in a rat model
    Author
    Secombe, KR;Ball, IA;Shirren, J;Wignall, AD;Finnie, J;Keefe, D;Avogadri-Connors, F;Olek, E;Martin, D;Moran, S;Bowen, JM;
    Year
    2018
    Journal
    Cancer Chemother. Pharmacol.
    Application
    WB
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