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에볼라 바이러스 EBOV (subtype Sudan, strain Gulu) GP1 / Glycoprotein HEK293 Cell Lysate (WB 양성대조군)

    데이터시트리뷰관련제품프로토콜
      Cells Transfected Lysate Product Information
    발현 숙주:Human Cells
    제품 설명:Human Cell lysate that Ebola virus EBOV (subtype Sudan, strain Gulu) GP1 / Glycoprotein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
    Sequence information:A DNA sequence encoding the Sudan ebolavirus (strain Gulu) GP (YP_138523.1) (Met1-Arg501) was expressed with a polyhistidine tag at the C-terminus.
    예상된 N 말단(N Terminal):Met 33
    분자량:The recombinant Sudan ebolavirus (strain Gulu) GP consists 480 amino acids and predicts a molecular mass of 52.5 kDa.
    :EBOV
      Transfected / Overexpression Cell Lysate Usage Gui
    준비 방법:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    품질 관리 검사:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
    안정성:Samples are stable for up to twelve months from date of receipt.
    권장 사용:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    저장:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
    신청 메모:Western blot (WB): Use at an assay dependent dilution.
    Other Applications: Not tested.
    Optimal dilutions/concentrations should be determined by the end user.
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    Cat No: 40094-V08H2L-300
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