Anti-Endoglin / CD105 Antibody

1/2
Price:
Size:
Number:

Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Endoglin / CD105 Antibody
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Endoglin / CD105
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Endoglin / CD105
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Endoglin / CD105 , and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:1500-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Endoglin / CD105 Antibody (Rabbit Polyclonal antibody) Images

Immunofluorescence staining of ENG in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ENG polyclonal antibody (1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.

Anti-ENG rabbit polyclonal antibody at 1:500 dilution

Lane A: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:70 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Endoglin / CD105 Antibody: Alternative Names

Anti-END Antibody; Anti-HHT1 Antibody; Anti-ORW1 Antibody

Endoglin / CD105 Background Information

Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait