Anti-Her2 / ERBB2 Antibody (PE)

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Anti-Her2 / ERBB2 Antibody (PE) (Rabbit Monoclonal antibody) General Information

Product name
Anti-Her2 / ERBB2 Antibody (PE)
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Her2 / ERBB2
Immunogen
Recombinant Human ErbB2 protein (Catalog#10004-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human ErbB2 / HER2 extracellular domain (rh ErbB2; Catalog#10004-H08H; NP_004439.2; Met 1-Thr 652) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Rabbit IgG Clone #511
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Conjugate
PE
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-Her2 / ERBB2 Antibody (PE) (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 5-20 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Her2 / ERBB2 Antibody (PE) (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of Human ErbB2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (PE-conjugated, 10 µg/ml) at 37℃ 1 hour. Positive staining was localized to plasma membrane.
Analysis of anti-human ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with PE Rabbit anti-human ErbB2.

Anti-Her2 / ERBB2 Antibody (PE): Alternative Names

Anti-CD340 Antibody; Anti-ENV Antibody; Anti-ENVW Antibody; Anti-ERVWE1 Antibody; Anti-HER-2 Antibody; Anti-HER-2/neu Antibody; Anti-HER2 Antibody; Anti-HERV-7q Antibody; Anti-HERV-W-ENV Antibody; Anti-HERV7Q Antibody; Anti-HERVW Antibody; Anti-HERVWENV Antibody; Anti-MLN 19 Antibody; Anti-MLN19 Antibody; Anti-NEU Antibody; Anti-NGL Antibody; Anti-TKR1 Antibody

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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