Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb

Price:
Size:
Number:

Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H1N1 Hemagglutinin / HA
Has cross-reactivity in ELISA with
H1N1 (A/Brisbane/59/2007) HA1
H1N1 (A/Solomon Islands/3/2006)
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/WSN/1933) HA
No cross-reactivity in ELISA with
H1N1 (A/California/07/2009) HA
H1N1 (A/California/04/2009) HA
H1N1 (A/California/04/2009) HA1
H1N1 (A/BrevigMission/1/1918) HA
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976)
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Anhui/1/2005) HA2
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/bar-headed goose /Qinghai/14/2008) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/Viet Nam/1203/2004) HA
H3N2 (A/Brisbane/10/2007) HA
Influenza B (B/Florida/4/2006) HA
H9N2 (A/Hong Kong/1073/99) HA
H7N7 (A/Netherlands/219/03) HA
H2N2 (A/Canada/720/2005) HA
Immunogen
Recombinant H1N1 (A/Brisbane/59/2007) HA protein (Catalog#11052-V08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Brisbane/59/2007) HA (Catalog#11052-V08H; ACA28844.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #2D10C9E2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N1 (A/Brisbane/59/2007) HA in WB. Using a DAB detection system, the detection limit for H1N1 (A/Brisbane/59/2007) HA is approximately 1 ng/lane under non-reducing conditions and reducing conditions.
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin / HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait