Anti-Her2 / ERBB2 Antibody

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Anti-Her2 / ERBB2 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Her2 / ERBB2 Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Her2 / ERBB2
Immunogen
Recombinant Human HER2 / ErbB2 protein (Catalog#10004-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HER2 / ErbB2 (rh HER2 / ErbB2; Catalog#10004-H08H; NP_004439.2; Met1-Thr652). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #03
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Her2 / ERBB2 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Her2 / ERBB2 Antibody (Mouse Monoclonal antibody) Images

Immunofluorescence staining of Human ERBB2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human ERBB2 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cell membrane.
Flow cytometric analysis of anti-Human ErbB2 on MCF-7 cells.

Anti-Her2 / ERBB2 Antibody: Alternative Names

Anti-CD340 Antibody; Anti-ENV Antibody; Anti-ENVW Antibody; Anti-ERVWE1 Antibody; Anti-HER-2 Antibody; Anti-HER-2/neu Antibody; Anti-HER2 Antibody; Anti-HERV-7q Antibody; Anti-HERV-W-ENV Antibody; Anti-HERV7Q Antibody; Anti-HERVW Antibody; Anti-HERVWENV Antibody; Anti-MLN 19 Antibody; Anti-MLN19 Antibody; Anti-NEU Antibody; Anti-NGL Antibody; Anti-TKR1 Antibody

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates
    Author
    Eletxigerra, U;Martinez-Perdiguero, J;Barderas, R;Pingarrón, JM;Campuzano, S;Merino, S;
    Year
    2016
    Journal
    Anal. Chim. Acta
  • para-Sulfonatocalix[4]arene stabilized gold nanoparticles multilayers interfaced to electrodes through host-guest interaction for sensitive ErbB2 detection.
    Author
    Wang, X;Du, D;Dong, H;Song, S;Koh, K;Chen, H;
    Year
    2017
    Journal
    Biosens Bioelectron
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