rat ICAM1 was immunoprecipitated using:
Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 µL anti-rat ICAM1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-rat ICAM1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 58 kDa
Observed band size: 116 kDa