Anti-ICAM-1 Antibody

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Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-ICAM-1 Antibody
Validated applications
ELISA,ELISA(Cap),FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human ICAM-1
No cross-reactivity in ELISA with
Human ICAM2
Human ICAM3
Human VCAM1
Human CD62E
Human CEACAM1
Human cell lysate (293 cell line)
Immunogen
Recombinant Human ICAM-1 protein (Catalog#10346-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54; Catalog#10346-H08H; Met 1-Glu 480; NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. ved.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
ICC-IF 1:20-1:100
FCM 1:25-1:100
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human ICAM1 / CD54 in ELISA pair set (Catalog: # SEK10346). In a sandwich ELISA, it can be used as capture antibody.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) Images

Immunofluorescence staining of Human ICAM1 in MSC cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with Mouse anti-Human ICAM1 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-α (50ng/ml,18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with purified human ICAM1 (10346-MM01), then a FITC-conjugated second step antibody.

Anti-ICAM-1 Antibody: Alternative Names

Anti-BB2 Antibody; Anti-CD54 Antibody; Anti-ICAM-1 Antibody; Anti-P3.58 Antibody

ICAM-1 Background Information

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
Full Name
intercellular adhesion molecule 1
Research Areas
References
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.

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