Influenza A H10N8 Hemagglutinin / HA Neutralizing Antibody

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Influenza A H10N8 Hemagglutinin / HA Neutralizing Antibody (Mouse Monoclonal antibody) General Information

Product name
Influenza A H10N8 Hemagglutinin / HA Neutralizing Antibody
Validated applications
Microneutralizaiton(MN),HemagglutininInhibition(HI) (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H10N8 Hemagglutinin / HA
Has cross-reactivity with H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin (Catalog # 11693-V08H) / H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin (Catalog # 40351-V08H1)
No cross-reactivity with H10N3 (A/mallard/Minnesota/Sg-00194/2007) Hemagglutinin (Catalog # 40184-V08B) in ELISA assay.
Immunogen
Recombinant Influenza A H10N8 (A/Jiangxi-Donghu/346/2013) Hemagglutinin / HA protein (Catalog#40359-V08B)
Preparation
It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant Influenza A H10N8 (A/Jiangxi-Donghu/346/2013) Hemagglutinin / HA. The antibody was produced using recombinant antibody technology.
Source
Monoclonal mouse (varialbe region) / human (kappa / IgG1 constant) chimeric antibody Clone #mh001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H10N8 Hemagglutinin / HA Neutralizing Antibody (Mouse Monoclonal antibody) Validated Applications

Notes
Hemagglutinin Inhibition (HI): This H10N8 HA protein Neutralizing Antibody can block the binding of the recombinant H10N8 HA protein to sialic acid and inhibit the hemagglutinin activity of recombinant H10N8 HA protein. The HI titer is 0.1-0.4 µg/mL When 1 unit of H10N8 HA protein (Catalog # 40359-V08B) added.
Microneutralizaiton (MN): This H10N8 HA protein Neutralizing Antibody can block the binding of the recombinant H10N8 HA protein to sialic acid and inhibit the hemagglutinin activity of recombinant H10N8 HA protein. The HI titer is 0.1-0.4 µg/mL When 1 unit of H10N8 HA protein (40359-V08B) added.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Influenza A H10N8 Hemagglutinin / HA Neutralizing Antibody (Mouse Monoclonal antibody) Images

The neutralization activity of H10N8 HA protein antibody is Measured by Microneutralization test in vitro. The pseudovirus microneutralizaiton (MN) test was performed on 293FT cells infected with H10N8 (A/Jiangxi-Donghu/346/2013) pseudovirus under the treatment of serial dilution of Neutralizing Antibody. The inhibition is 99.3% under 1 μg/mL influenza A H10N8 HA Neutralizing Antibody (Catalog # 40359-mh001) with high titer H10N8 pseduotype virus.

Hemagglutinin / HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37

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