S100A4 Protein, Mouse, Recombinant (His Tag)

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S100A4 Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mouse S100A4 (NP_035441.1) (Met1-Lys101) was expressed with a polyhistidine tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
His
Molecule Mass
The recombinant mouse S100A4 consists of 117 amino acids and predicts a molecular mass of 13.8 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A4 Protein, Mouse, Recombinant (His Tag): Images

S100A4 Protein, Mouse, Recombinant (His Tag): Alternative Names

18A2 Protein, Mouse; 42a Protein, Mouse; Capl Protein, Mouse; FSp1 Protein, Mouse; metastasin Protein, Mouse; Mts1 Protein, Mouse; PeL98 Protein, Mouse; pk9a Protein, Mouse

S100A4 Background Information

S1A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S1 proteins containing two EF-hand calcium-binding motifs. In humans at least 2 S1 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S1A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S1A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S1A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
Full Name
S100 calcium binding protein A4
References
  • Ambartsumian N. et al., 1995, Gene. 159: 125-30.
  • Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22.
  • Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8.
  • Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30.
  • Boye K. et al., 2008, Int J Cancer. 123: 1301-10.
  • Garrett SC. et al., 2006, J Biol Chem. 281: 677-80.
  • Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
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